A2103
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 | Immunoblotting Rat skeletal muscle and HeLa total cell extract were separated on SDS-PAGE and probed with monoclonal AIA to Actin N-Terminal (RB) (Cat. No. A2103). The antibody was developed with HRP APA Rabbit conjugate (Cat. No. A0545) and ECL substrate. Antibody concentration of Rat skeletal muscle 2.0 ng/mL. Antibody concentration of HeLa total cell extract 0.5 μg/mL. |
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A2103
Sigma
Anti-Actin, N-terminal antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
Description
| Immunogen | synthetic actin N-terminal nonapeptide conjugated to KLH. |
| Other Notes | To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide. |
Properties
| antibody form | affinity isolated antibody |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt 42 kDa |
| species reactivity | mouse, frog, human, chicken, rat |
| application(s) | immunoblotting: 0.5-1 μg/mL using whole extract of the human epitheloid carcinoma HeLa cell line. |
| immunoblotting: 2-4 ng/mL using whole extract of rat skeletal muscle | |
| immunocytochemistry: 1-2 μg/mL using cultured chicken fibroblasts | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2-4 μg/mL using sections of human appendix, mouse heart, and frog skeletal muscle | |
| Gene Information | human ... ACTA1(58), ACTA2(59), ACTB(60), ACTC1(70), ACTG1(71), ACTG2(72) |
| rat ... Acta1(29437), Acta2(81633), Actb(81822), Actc1(29275), Actg1(287876), Actg2(25365) | |
| mouse ... Acta1(11459), Acta2(11475), Actb(11461), Actc1(11464), Actg1(11465), Actg2(11468) | |
| shipped in | wet ice |
| storage temp. | −20°C |
Safety
| WGK Germany | NWG |
References
| reference | Botkjaer, A., et al., A role for docosahexaenoic acid–derived neuroprotectin D1 in neural cell survival and Alzheimer disease J. Clin. Invest. 115, 2774-2783, (2005) Abstract |
| Aggeli, I., et al., CoCl2 induces protective events via the p38-MAPK signalling pathway and ANP in the perfused amphibian heart. J. Exp. Biol. 210, 2267-2277, (2007) Abstract | |
| Lombard, D.B., et al., A role for the NAD-dependent deacetylase Sirt1 in the regulation of autophagy Proc. Natl. Acad. Sci. U. S. A. 105, 3374-3379, (2008) Abstract |
